Fluorescent peptide indicator displacement assay for monitoring interactions between RNA and RNA binding proteins.
نویسندگان
چکیده
This paper describes a sensitive, non-destructive displacement assay, using a fluorescent peptide indicator, for real-time monitoring of the interactions between RNA and RNA binding proteins (RBPs). The developed fluorescent peptide indicators, each containing a mid-sequence fluorophore unit, allowed sensing of target RNA and RNA-RBP interactions through changes in fluorescence intensity. We anticipate that this assay will open up new possibilities for meaningful studies of RNA-RBP interactions.
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عنوان ژورنال:
- Molecular bioSystems
دوره 9 5 شماره
صفحات -
تاریخ انتشار 2013